Increasing Microalgae Biomass Production Navicula Sp. with Carbon Dioxide (CO2) in The Photobioreactor

Abstract

The use of microalgae as an alternative energy source has many advantages, including fast growth, does not require large areas of land, and high lipid production. Utilization of microalgae as biofuels requires high total microalgae biomass production. One of the ways to increase biomass is the CO2 content in the media. This study aims to determine the optimum CO2 concentration for Navicula sp. microalgae. thus obtaining high biomass. The study was conducted using a randomized block design with CO2 concentration treatment, namely; P1 (0% CO2+ 10% harvest); P2 (10% CO2+ 10% harvest); P3 (20% CO2+ 10% harvest); P4 (0% CO2+ 20% harvest); P5 (10% CO2+ 20% harvest); and P6 (20% CO2+ 20% harvest). The working methods in this study included making f/2 media, autobioreactor sterilization, inoculation, cultivation in the photobioreactor and sampling. The highest abundance of cells and biomass was obtained in the P3 treatment (20% CO2+ 10% harvest) and P2 (10% CO2+ 10% harvest) with yields of 4.2 x 106 cells/m and 1.7 g/L/day, while the total The highest biomass production for 11 days was obtained in the P2 treatment (10% CO2+ 10%) which was 18.14 g/L/11 days. Statistical analysis of the One-Way Anova test showed that giving a CO2 concentration of 10% had an effect on increasing the biomass production of Navicula sp.